The new service called Amazon Business will offer enterprises the opportunity to shop online for productions ranging from computer and lab equipment to education and food service supplies.
It will offer special services for businesses including a dedicated customer support team, Amazon said.
"We've heard from business customers that they love the convenience of shopping online, and want an experience at work that is similar to how they shop at home," said Amazon vice president Prentis Wilson.
"Amazon Business delivers a new and expanded marketplace that brings the selection, convenience and value of Amazon to business customers, manufacturers and sellers with the additional selection, features and back-end integration businesses need to save time and money."
The move comes with businesses increasingly going online for managing their day-to-day supply needs.
Forrester Research estimates that business to business (B2B) e-commerce will top $1.1 trillion and account for 12.1 percent of all US sales in the segment by 2020.
"B2B buyers are now expecting B2C-like (retail) customer experiences online, and they're growing increasingly impatient with B2B sellers that don't provide it," says Forrester analyst Andy Hoar.Get your daily dose of tech news, reviews, and insights, in under 80 characters on Gadgets 360 Turbo. Connect with fellow tech lovers on our Forum. Follow us on X, Facebook, WhatsApp, Threads and Google News for instant updates. Catch all the action on our YouTube channel.